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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of this gene is observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was observed to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and performs a critical job in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence information and facts is not really however obtainable.

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Blocking peptides are peptides that bind exclusively for the target antibody and block antibody binding. These peptide typically is made up of the epitope acknowledged from the antibody.

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This guidebook outlines even further attainable optimization of cellular and immunoassay parameters to be certain the very best benefits are...

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This guideline outlines further probable optimization of mobile and immunoassay parameters to ensure the best possible benefits are...

Antibodies certain to the blocking peptide no more bind to your epitope over the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is certain.

Goal Info Cyclin E belongs to your really conserved cyclin spouse and children, whose members are characterised by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins operate as regulators of CDK kinases. Distinctive cyclins exhibit distinctive expression and degradation patterns which add for the temporal coordination of each and every mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates for the G1-S stage boundary and is degraded as cells development as a result of S stage.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both antibodies...

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